https://ogma.newcastle.edu.au/vital/access/ /manager/Index en-au 5 https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:45259 sn-glycero-2-phosphocholine and cholesterol were prepared according to the method of dried lipid film hydration. Ligands were conjugated with the surface of liposomes using optimized methods to maximize conjugation efficiency. The liposomes were characterized for particle size, ligand conjugation, drug encapsulation, liposome stability, specificity of binding, cellular internalization, mechanistic pathway of cellular uptake, and cellular toxicity. Results: Both OTR-Lipo and ATO-Lipo showed significant and specific binding to OTRs in a concentration-dependent manner compared to all control groups. There was no significant difference in binding values between OTR-Lipo and ATO-Lipo across all concentrations evaluated. In addition, OTR-Lipo (81.61%±7.84%) and ATO-Lipo (85.59%±8.28%) demonstrated significantly increased cellular internalization in comparison with rabbit IgG immunoliposomes (9.14%±1.71%) and conventional liposomes (4.09%±0.78%) at 2.02 mM phospholipid concentration. Cellular association following liposome incubation at 4.05 mM resulted in similar findings. Evaluation of the mechanistic pathway of cellular uptake indicated that they undergo internalization through both clathrin- and caveolin-mediated mechanisms. Furthermore, cellular toxicity studies have shown no significant effect of both liposomal platforms on cell viability. Conclusion: This study further supports OTRs as a novel pharmaceutical target for drug delivery. OTR-targeted liposomal platforms may provide an effective way to deliver existing therapies directly to myometrial tissue and avoid adverse effects by circumventing non-target tissues.]]> Wed 26 Oct 2022 19:46:49 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:11010 Wed 11 Apr 2018 11:06:17 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:31147 Thu 30 Jan 2020 10:44:09 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:19928 Sat 24 Mar 2018 08:03:47 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:19398 b)pyrazine) (pC₆TP) and [6,6]-phenyl-C₆₁-butyric acid methylester (PCBM) have been prepared and studied as near infra-red (NIR) organic photonic devices. Photocurrent contributions from both components are observed and show a photoresponse down to approximately 1 eV. The performance of the devices as a function of annealing time is investigated. A model for the effect of structure on the NIR response of these devices is developed and highlights the ambipolar nature of pC₆TP as a charge transport material.]]> Sat 24 Mar 2018 07:52:04 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:45396 Mon 29 Jan 2024 18:39:17 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:50649 Mon 29 Jan 2024 18:35:00 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:48938 Mon 29 Jan 2024 18:22:46 AEDT ]]>